K. P. Daniel Yip, Ph.D.
Biographical Sketch | Interests | Current Research | Major Publications
Ph.D., University of Southern California, 1991
Office: MDC 3034
E-Mail: dyip@hsc.usf.edu
Phone: (813) 974-7111
FAX: (813) 974-3079
Biographical Sketch
Positions and Employment
- 1992-1993 American Heart Association Postdoctoral Fellowship, UCLA
- 1993-1999 Research Assistant Prof. of Physiology, Brown University
- 1999-2003 Assistant Prof. of Physiology & Biophysics, USF
- 2003-present Associate Prof. of Physiology & Biophysics, USF
Other Experience
- 2003-Present Editorial Board : American Journal of Physiology Renal Physiology
Honors
- 1992 Clifford and Evelyn Cherry Fellowship Award , American Heart Association Greater Los Angeles Affiliate
Interests
Dynamics of intracellular Ca2+ in renal epithelium and smooth muscle
Pressure diuresis and hypertension.
Current Research
My research involves the development and application of laser-based videomicroscopy, confocal fluorescence microscopy, and flash photolysis to study renal cellular function in situ and in vitro. Currently we are investigating the signal transduction process and mechanism of aquaporin-2 trafficking in intact inner medullary collecting duct, role of integrins in mechanical signal transduction of renal blood autoregulation, and cellular mechanisms of pressure natriuresis and its relationship with the development of hypertension.
Project of graduate student (Lavanya Balasubramanian)
The aims of this project are to test whether integrins serve as mechanotransducers and trigger local calcium transients (Calcium sparks) in freshly isolated rat renal vascular smooth muscle cells, and whether integrins are required for myogenic response in perfused rat afferent arterioles. Coated paramagnetic beads are used to apply controlled mechanical force to freshly isolated renal smooth muscle cells. Calcium sparks are detected using high-speed linescan confocal imaging. Perfused afferent arteriole is used to study the mechanism of integrin-mediated vasoconstriction.
Keywords
confocal fluorescence microscopy, videomicroscopy, immunofluorescence, integrins, calcium oscillations, Calcium sparks, intracellular pH, Na+/H+ exchangers, pressure natriuresis, hypertension, renal hemodynamics, tubuloglomerular feedback, myogenic response.
Major Publications
Chan W.L, N.-H. Holstein-Rathlou, and K. P. Yip. Integrin mobilizes intracellular Ca 2+ in renal vascular smooth muscle cells. Am. J. Physiol. Cell Physiol. 280 : C593-C603, 2001.
Yip, K.P. Coupling of intracellular calcium mobilization and apical exocytosis in perfused rat inner medullary collecting duct. J. Physiol. (Lond.) 538 : 891-899, 2002.
Yip, K.P., S. Tsuruoka, G.J. Schwartz, and I. Kurtz. Role of NBC3 in mediating bicarbonates transport and pHi regulation in the outer medullary collecting duct. Am. J. Physiol. Renal Physiol. 283 : F1098-F1104, 2002.
Yip K.P. and I. Kurtz. Confocal fluorescence microscopy measurement of pH and calcium in living cells. Meth. Cell Bio. 70 : 417-427, 2002.
Zhang, W.-M., K. P. Yip, M.-J. Lin, L.A. Shimoda, W.-L. Li, and J. S. K. Sham. Endothelin-1 activates Ca2+ sparks in pulmonary arterial smooth muscle cells: local Ca2+ signaling between inositol triphosphate- and ryanodine-receptors. Am. J. Physiol. Lung Cell. Mol. Physiol. 285 ;L680-L690, 2003.
Walstead C. and K. P. Yip Acute arterial hypertension inhibits proximal tubular fluid reabsorption in normotensive rat but not in SHR. Am. J. Physiol. Regulatory Integrative Comp. Physiol. 286 : R726-R733, 2004.
Lin, M.-J.,G. P. H. Leung, W.-M. Zhang, X.-R. Yuang, K. P. Yip, C.-M. Tse, and J.S.K. Sham. Chronic hypoxia-induced upregulation of store-operated and receptor-operated Ca2+ channels in pulmonary arterial smooth muscle cells-a novel mechanism of hypoxic pulmonary hypertension. Circ. Res. 95: 496-505, 2004.
Chon, K.H., R. Raghavan, Y.-M. Chen, D.J. Marsh, and K.P. Yip. Interactions of TGF-dependent and myogenic oscillations in tubular pressure. Am. J. Physiol. Renal Physiol. 288 : F298-F307, 2005.
Yip, K.P. Flash photolysis of caged nitric oxide inhibits proximal tubular fluid reabsorption in free flow nephron. Am. J. Physiol. Regulatory Integrative Comp. Physiol. doi:10.1152/ajpregu.00610.2004